Chd1 removes nucleosomal barriers to transcriptional elongation 10 and is required for the optimal activity of RNA Pol I and II 3. Chd1 is an ATP-dependent chromatin remodeler that binds specifically to H3K4me3 and is found at sites of active transcription 8, 9. Some of the players implicated in promoting hypertranscription are the transcription factors Myc and Yap/Taz, the RNA Polymerase regulator pTEFb and the chromatin remodeler Chd1 (reviewed in Percharde et al. It is expected that hypertranscription involves a coordinated interplay between activating transcription factors, chromatin remodelers, and RNA Polymerases. The molecular regulation of hypertranscription, or even how it differs from general transcriptional regulation, remains poorly understood. Hypertranscription has been documented to occur and play critical roles in embryonic stem (ES) cells 2, the post-implantation epiblast 3, emergence of definitive hematopoietic stem cells 4, primordial germ cells 5, and neurogenesis 6, and may take place in other settings during development, regeneration, and disease 1, 7. Hypertranscription is masked by most transcriptional profiling approaches, but has attracted renewed interest recently.
One way that stem/progenitor cells cope with this demand is to enter a state of hypertranscription, which involves a global elevation of nascent transcriptional output 1.
Proliferating stem and progenitor cells are net generators of new cellular biomass and therefore have high biosynthetic demand. These results reveal a vulnerability of hypertranscribing stem cells to accumulation of endogenous DNA breaks, with important implications for developmental and cancer biology. Genes prone to DNA breaks in Chd1 KO ES cells are longer genes with GC-rich promoters, a more labile nucleosomal structure and roles in chromatin regulation, transcription and signaling.
Chd1 interacts with several DNA repair factors including Atm, Parp1, Kap1 and Topoisomerase 2β and its absence leads to an accumulation of DSBs at Chd1-bound Pol II-transcribed genes and rDNA. Here we report a novel role for Chd1 in protecting genome integrity at promoter regions by preventing DNA double-stranded break (DSB) accumulation in ES cells. The chromatin remodeler Chd1 mediates hypertranscription in pluripotent cells but its mechanism of action remains poorly understood. Some individuals/departments might want to use a fee-based service such as GMR Transcription (favored in the Curry School) or Rev as they reason time and staff are also money.Stem and progenitor cells undergo a global elevation of nascent transcription, or hypertranscription, during key developmental transitions involving rapid cell proliferation. Having everyone in the class/department consistently use the same transcription tool can help create a higher level of support as members can ask others for help solving problems or allowing another person to step into the transcription role if someone graduates.
Hypertranscribe software how to#
If a researcher elects to use software, they will need to factor in the time it takes to learn how to use the software as well as the time it will take to transcribe the interviews. The decision on what transcription software to use comes down to time, money, deadlines, tech support needs, and requirements of the class/ department.
Hypertranscribe software for mac#
Free version good for Mac PC free version has a lot of adware. One person doesn’t feel the need to use a pedal another uses the AltoEdge pedal.
Hypertranscribe software plus#
MAXQDA Standard: (also Plus and Analytics Pro)Įxpress Scribe (Mentioned by several. Listen & Write: Elefant software (they also have a lot of free accessibility tools): and. How to digitally record and transcribe an interview: Speechlogger: (This is the program Google uses for Voice Typing in Google Docs) However, there are a variety of other transcription software solutions including:ĭragon (expensive, but word-of-mouth is very positive for dictation) It has a Speech Input facility to create text. Here are directions for how to customize it and do punctuation: Īnd there’s Read&Write Gold (11.5), which we have free access to here at UVa.
Sure, if you have a Google account and use the Chrome browser, a fast-and-simple solution that does not require any additional downloading is the “Voice Typing” feature under “Tools” in Google Docs.
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